FastQCFastQC Report
Sat 18 Mar 2023
SRR4895485_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895485_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650292
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA1652050.0143.736952
TTCACGC1652900.0143.663041
CACGCAT540750.0143.614333
ACGCATG171250.0143.389594
ACGCAAT144850.0142.950014
ACGCAGT158700.0142.851034
CACGCAG506150.0142.827673
ACGCATA159200.0142.763924
ACGCATT167100.0142.386784
CGCATGA48600.0142.265325
CGCATAG51300.0142.070535
CACGCAA446450.0142.024373
CGCAATA41900.0141.318085
CGCATAA46750.0141.27755
CGCATAT57000.0141.116855
ACGCATC46700.0140.966574
CGCAATG49750.0140.85685
CGCATTA50650.0140.626665
CGCAAGT47750.0140.578925
ACGCAGA140600.0140.567614
Produced by FastQC (version 0.11.5)