FastQCFastQC Report
Sat 18 Mar 2023
SRR4895485_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895485_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650292
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA133950.8116745400207964No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG43960.2663771017492662No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC30550.18511875474158512No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC28650.17360564069873696No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG22350.1354305783461351No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC18880.11440399638367028No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC18820.11404042436126455No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA18450.11179839688976255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA249250.0136.480448
AAGCAGT250200.0136.171941
GTGGTAT251350.0135.36886
TGGTATC252300.0134.916147
GTATCAA253450.0134.218789
AGTGGTA257000.0132.532795
AGCAGTG257700.0132.320592
CAGTGGT258600.0131.824114
GCAGTGG263200.0129.582873
TCAACGC249850.027.93909610-14
ATCAACG249950.027.84154710-14
AGAGTAC251350.027.65261515-19
TACATGG251350.027.6306820-24
CAGAGTA252200.027.57653615-19
GTACATG252850.027.51231620-24
AGTACAT253600.027.39688720-24
CAACGCA256800.027.21658710-14
GCAGAGT258050.026.9625315-19
GAGTACA251500.026.96162820-24
CGCAGAG259350.026.8218315-19
Produced by FastQC (version 0.11.5)