FastQCFastQC Report
Sat 18 Mar 2023
SRR4895484_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895484_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650787
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTA1655800.0143.442752
TGGTAGC277450.0143.181474
TGGTATC276500.0142.944924
GTGGTAG514950.0142.931583
GGTATCC194050.0142.880585
GTGGTAT587600.0142.791933
TGGTAAC195750.0142.338014
GTGGTAA431550.0142.264543
GGTAGCC215600.0142.079715
GTGGTAC131000.0142.069343
TGGTGGT1673750.0141.90441
GTATCCG68700.0141.891946
GTAGCCG71700.0141.473516
GTAGCCT61800.0141.436976
GTAACCG50050.0141.150566
TGGTACC93300.0141.028614
GGTAACC147950.0140.380575
GTATCCT57400.0140.372276
TAGCCGT25500.0139.931887
GTATCCA46950.0139.897636
Produced by FastQC (version 0.11.5)