FastQCFastQC Report
Sat 18 Mar 2023
SRR4895484_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895484_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650787
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA128280.7770838999822509No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG48420.2933146432580339No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT36510.2211672372026191No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC36340.22013742536135797No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA23980.14526404678495772No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC21140.12806013131918292No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT20710.12545531313246347No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCGGTGGCTCAC20240.12260818627721203No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA19640.11897355624923144No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT19280.11679277823244308No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC19150.11600527505971395No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT18270.11067448435200908No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG17570.10643408265269838No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC17320.10491965347437314No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA16880.1022542581205207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT232500.0136.561116
GGTATCA232600.0136.50248
AAGCAGT233300.0136.078611
TGGTATC234600.0135.400047
GTATCAA234300.0135.358419
AGTGGTA236800.0134.081335
CAGTGGT239550.0132.63224
AGCAGTG240400.0131.849982
GCAGTGG245250.0129.359993
CCGTACC900.003763224531.983947
ATCAACG231000.028.0503310-14
TCAACGC232100.027.96710-14
AGAGTAC231750.027.9222915-19
CAGAGTA234100.027.63584515-19
TACATGG234250.027.62517720-24
CAACGCA236550.027.44088210-14
GTACATG235650.027.43661920-24
GCAGAGT238400.027.1615315-19
AGTACAT238350.027.0895820-24
CGCAGAG239150.027.08236515-19
Produced by FastQC (version 0.11.5)