FastQCFastQC Report
Sat 18 Mar 2023
SRR4895483_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895483_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2577001
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTCA2583250.0143.57392
TGGCTTC2585000.0143.47671
GCTTCAG810250.0143.096133
GCTTCAT845850.0142.994783
GCTTCAA690200.0142.962893
CTTCATG280150.0141.867174
CTTCAGT264700.0141.203524
GCTTCAC250500.0140.991963
CTTCAAT237300.0140.616684
CTTCAGA225450.0140.602544
CTTCAGG272700.0140.570794
CTTCAAG227750.0140.28854
CTTCATA252900.0139.027954
CTTCATT259150.0136.841234
TTCATAG88200.0136.08965
CTTCACG72500.0136.080834
TTCAGAT82200.0135.342795
TTCATGG104250.0134.60345
TTCATGA79500.0134.508325
TTCAATG88350.0134.392465
Produced by FastQC (version 0.11.5)