FastQCFastQC Report
Sat 18 Mar 2023
SRR4895483_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895483_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2577001
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA174700.6779197990221968No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG88750.34439257105449317No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC61040.23686447929201424No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT51260.19891338808172757No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA34370.1333720863903429No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC34230.1328288192360034No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT29670.1151338319232317No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA29610.1149010031428005No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC29290.11365924964716739No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT27780.10779972533964868No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT27250.10574307111250636No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT27080.10508338956795127No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC26340.10221183460929972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA401150.0135.340338
AAGCAGT401850.0135.195021
GTGGTAT402550.0134.72666
TGGTATC404700.0134.153147
GTATCAA404950.0134.052549
AGTGGTA409200.0132.466775
CAGTGGT412350.0131.62944
AGCAGTG413500.0131.316392
GCAGTGG421450.0128.822223
TCAACGC401300.027.67852610-14
ATCAACG400450.027.66178910-14
AGAGTAC402950.027.45897315-19
TACATGG404800.027.30247520-24
CAGAGTA406300.027.22194115-19
GTACATG408050.027.09913320-24
GAGTACA402050.026.85535220-24
GCAGAGT411800.026.84088315-19
CAACGCA414150.026.84059110-14
AGTACAT412750.026.73125520-24
ACATGGG410850.026.5885320-24
Produced by FastQC (version 0.11.5)