FastQCFastQC Report
Sat 18 Mar 2023
SRR4895482_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895482_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213985
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAACA1220600.0143.128621
GAACAAT443850.0142.7133
GGAACAA1233050.0141.683472
GAACAAG390200.0141.404723
AACAATG148650.0139.556094
ACAATAG43500.0138.12315
AACAAGT125550.0137.436334
ACAAGTG38100.0135.413915
ACAATGA43050.0134.88685
ACAATGG51500.0134.830815
GAACAAC121850.0134.40513
ACAATGT53950.0133.909485
AACAATA137150.0133.891784
ACAAGTA42450.0133.572665
CAATATG17950.0132.287416
ACAATTG49200.0131.919975
CAATGTG17550.0131.612466
CAATGAG15400.0130.362476
ACAAGAT39300.0129.814365
GAACAAA307050.0129.640933
Produced by FastQC (version 0.11.5)