FastQCFastQC Report
Sat 18 Mar 2023
SRR4895482_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895482_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213985
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA109030.898116533565077No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG34090.28081071841909083No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC21810.1796562560492922No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA16970.13978755915435528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA209000.0137.16218
GTGGTAT210100.0136.512486
TGGTATC210750.0136.023157
GTATCAA210800.0135.990899
AAGCAGT210600.0135.976581
AGTGGTA214950.0133.298375
CAGTGGT215500.0133.091754
AGCAGTG217050.0131.935822
GCAGTGG223050.0128.419053
TCAACGC208200.028.11859110-14
ATCAACG208150.028.07693510-14
AGAGTAC209050.027.9078615-19
CAACGCA210000.027.8844310-14
GTACATG209700.027.75751120-24
CAGAGTA210600.027.72296315-19
AGTACAT210850.027.6061220-24
CGCAGAG211750.027.59279615-19
TACATGG210900.027.5722720-24
GAGTACA208600.027.35864320-24
GCAGAGT215500.027.13936415-19
Produced by FastQC (version 0.11.5)