FastQCFastQC Report
Sat 18 Mar 2023
SRR4895481_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895481_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723686
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAGAG726850.0143.271441
GAAGAGA731750.0142.312072
AAGAGAT251100.0140.953543
AAGAGAG232500.0138.21073
AAGAGAC77050.0136.901183
AAGAGAA198200.0131.489383
AGAGATG89950.0128.306634
AGAGATT78350.0128.01764
AGAGACT20250.0127.915554
AGAGATA81850.0126.5871664
AGAGAGT75000.0126.2526554
AGAGACG22750.0126.193614
AGAGACC23500.0125.8403244
GAGATGT33650.0124.232995
AGAGAAT69750.0123.376624
AGAGATC28100.0123.164244
GAGATTA23500.0123.08475
GAGATTG28950.0122.53055
GAGACTC3300.0122.101215
GAGAGTG26950.0121.7452245
Produced by FastQC (version 0.11.5)