FastQCFastQC Report
Sat 18 Mar 2023
SRR4895481_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895481_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723686
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67010.9259540740044715No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCAGTGGCTCA20170.27871203809386946No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA13980.1931777041424043No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC13870.19165770790094044No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAT13630.18834135246501935No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGGCGCGGTGGCTCACG12900.17825410468075933No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA11980.16554140884306176No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT11960.16526504589006835No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT11480.15863233501822613No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC10860.15006508347542996No Hit
CACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAG10780.14895963166345624No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA10610.14661054656301215No Hit
AGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCAG9990.13804329502021595No Hit
TGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCA9350.12919968052442632No Hit
CAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGT9310.1286469546184395No Hit
AGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTC9190.12698877690047894No Hit
AGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGG9120.12602150656500197No Hit
GTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAG8740.12077061045812686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT8730.12063242898163015No Hit
ATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCAGAGCTGCCCTCTC8700.12021788455214003No Hit
AGACGCTTTCAGTGGGTTGACGGCAGCCGCTGGAACTTTGCGTACTGGGC8570.11842152535768274No Hit
CATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCAGAGCTGCCCTCT8470.11703971059271563No Hit
CCTGGTCCCGCGGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTAC8440.11662516616322549No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCGGTGGCTCAC8240.11386153663329122No Hit
ACCAGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCCTGTGTACCCGAGGA8230.11372335515679452No Hit
AGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGC8010.11068336267386683No Hit
CCTGTGTACCCGAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGAC7970.11013063676788No Hit
GAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTC7970.11013063676788No Hit
CCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCC7960.10999245529138327No Hit
GAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGT7800.10778155166743589No Hit
GTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCC7700.10639973690246875No Hit
CTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCAGAGCTGCCCTCTCCT7620.10529428509049506No Hit
GGCAGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCG7590.10487974066100492No Hit
CTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCAGAGC7590.10487974066100492No Hit
GCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGT7550.10432701475501806No Hit
TTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCAGAGCT7540.10418883327852134No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA7490.10349792589603778No Hit
CTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7320.10114884079559366No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA7320.10114884079559366No Hit
CTACTGAGCTGGTCCCAGCCAGCAGTTCAGAGCTGCCCTCTCCTGGGCAG7260.1003197519366134No Hit
GTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCAGAGCTGCCCTCTCCTGG7260.1003197519366134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT106400.0136.771031
GGTATCA106250.0136.76248
GTGGTAT106550.0136.242236
GTATCAA107200.0135.751829
AGTGGTA107150.0135.54655
TGGTATC107400.0135.297997
CAGTGGT108800.0133.623184
AGCAGTG109300.0132.94452
GCAGTGG113750.0127.74363
ATCAACG106300.028.08437510-14
TCAACGC106900.027.9132810-14
AGAGTAC106000.027.9058515-19
CAGAGTA106050.027.8791215-19
GTACATG106500.027.84780120-24
TACATGG106450.027.77973620-24
AGTACAT107550.027.52238320-24
CAACGCA108550.027.47572710-14
GCAGAGT108400.027.27472915-19
GAGTACA106100.027.20647620-24
AACGCAG109600.027.17310110-14
Produced by FastQC (version 0.11.5)