FastQCFastQC Report
Sat 18 Mar 2023
SRR4895480_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895480_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3080268
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCAC3092550.0143.326421
CTTCACA3093100.0143.300932
TTCACAG970550.0142.70523
TCACATG323700.0142.63184
TTCACAT1009650.0142.537633
TCACAGT316200.0142.442464
TCACATA291550.0142.171264
TCACAGG324600.0140.684744
TCACAAT288700.0140.534294
TCACAGA264100.0140.520584
TCACATT316850.0140.379044
TCACAAG281000.0139.827594
TTCACAA853050.0139.22613
CACATAG99400.0137.818515
TTCACAC305500.0137.68123
CACATGA93700.0137.371865
TCACAGC78100.0136.805154
TCACACG82900.0136.781924
CACATGT118950.0136.700785
CACAGTG104900.0136.696955
Produced by FastQC (version 0.11.5)