FastQCFastQC Report
Sat 18 Mar 2023
SRR4895480_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895480_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3080268
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA272240.8838192001475197No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG75350.24462157188919925No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC56190.18241919209627214No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA33410.10846458814622623No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC32670.10606219978261631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT565800.0137.375371
GGTATCA567200.0136.941978
GTGGTAT568100.0136.788366
TGGTATC569050.0136.597937
GTATCAA572250.0135.695769
AGTGGTA575800.0134.971625
CAGTGGT577850.0134.617344
AGCAGTG582050.0133.540052
GCAGTGG590700.0131.584533
TCAACGC566700.028.04498110-14
ATCAACG566450.028.0141610-14
AGAGTAC570450.027.8129515-19
CAGAGTA572150.027.73282415-19
CAACGCA573650.027.73029510-14
TACATGG571150.027.69220-24
GTACATG572050.027.67611720-24
GCAGAGT577750.027.47896615-19
CGCAGAG578250.027.47760615-19
AGTACAT576750.027.438120-24
AACGCAG580950.027.3768910-14
Produced by FastQC (version 0.11.5)