FastQCFastQC Report
Sat 18 Mar 2023
SRR4895479_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895479_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2846598
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA2848950.0143.781852
CGTCTAG885150.0143.618563
CGTCTAA761100.0143.562733
TCCGTCT2853850.0143.534961
CGTCTAT939450.0143.520113
CGTCTAC268850.0142.859313
GTCTAAG249550.0142.69214
GTCTAGG286400.0142.646914
GTCTATG308300.0142.222724
GTCTAGT294600.0142.120134
GTCTATA266600.0141.932894
GTCTAAT266150.0141.79444
GTCTAGA247100.0141.60274
GTCTACG76800.0140.624014
TCTAGTA92550.0139.938165
GTCTATT301050.0139.123024
TCTATGA84150.0139.029335
GTCTAAA212750.0138.762474
TCTAGAT87000.0138.610085
GTCTATC81250.0138.589724
Produced by FastQC (version 0.11.5)