FastQCFastQC Report
Sat 18 Mar 2023
SRR4895478_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895478_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263152
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA100.007255848143.868718
CACGATG151.267933E-4143.86877
GCATACA151.267933E-4143.86876
TATCAGC263300.0143.786741
TCAGCAG82050.0143.693363
ATCAGCA263550.0143.650342
TCAGCAT85900.0143.453
CAGCATG27800.0143.092444
CAGCATA25400.0142.452674
TCAGCAA71700.0141.862183
AGCATGG10150.0141.742555
TCAGCAC25300.0141.309773
AGCATAA8200.0141.236975
AGCATAT8000.0141.171175
AGCATGA7500.0140.991335
CAGCAAT24900.0140.690894
AGCAGTA8300.0140.4025
CAGCAAG23700.0140.226464
CAGCAGT28050.0140.021964
CAGCATT26650.0139.280034
Produced by FastQC (version 0.11.5)