FastQCFastQC Report
Sat 18 Mar 2023
SRR4895478_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895478_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263152
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27061.0283030339879613No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA8180.31084696297197056No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT4690.1782239922174257No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGAATCCAGTGTTCTGTC3220.12236274092539673No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT2680.10184228126710038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41400.0137.150048
AAGCAGT41550.0136.68091
GTGGTAT41700.0136.50856
AGTGGTA41650.0136.49965
TGGTATC41750.0136.345027
GTATCAA42100.0135.040579
CAGTGGT42300.0134.572224
AGCAGTG42650.0133.324462
GCAGTGG43900.0129.528213
CGGAACG255.383208E-428.78586475-79
TCAACGC41300.028.2282710-14
ATCAACG41250.028.22759210-14
AGAGTAC41300.028.1585715-19
GTACATG41750.027.9314420-24
CAACGCA41800.027.92504110-14
CAGAGTA41750.027.88953815-19
TACATGG41850.027.830320-24
AACGCAG41950.027.75657310-14
AGTACAT42050.027.73216820-24
GCAGAGT42150.027.55657215-19
Produced by FastQC (version 0.11.5)