FastQCFastQC Report
Sat 18 Mar 2023
SRR4895477_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895477_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597960
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACAT1599800.0143.7061
CCACATA1601600.0143.544482
CACATAG502450.0143.457173
CACATAT510450.0143.22413
ACATAGG172600.0142.496984
CACATAA432700.0142.342863
ACATATG170250.0142.182244
ACATAGT156350.0142.174
CACATAC163000.0141.842273
CATAGGT56550.0141.837045
ACATAGA140150.0141.203314
ACATAAG146650.0141.02734
ACATAAT148350.0140.574984
CATATGA46950.0140.348345
ACATACG44300.0140.299914
ACATATA150250.0140.233674
CATAGAT48400.0140.156665
CATATGG60700.0139.96155
CATAGAG46500.0139.540735
CATAGGA49550.0139.5175
Produced by FastQC (version 0.11.5)