FastQCFastQC Report
Sat 18 Mar 2023
SRR4895477_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895477_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597960
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA112090.7014568574933039No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG39430.2467521089388971No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC28230.17666274499987483No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA24210.15150566972890434No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC23640.147938621742722No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA21430.13410848832261132No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT19920.12465894014868958No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT19370.12121705174096974No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA18040.11289393977321083No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC17740.11101654609627273No Hit
CACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAG17170.10744949811009037No Hit
TGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCA16720.10463340759468323No Hit
CAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGT16390.10256827455005131No Hit
AGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCAG16370.10244311497158878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203050.0136.625278
GTGGTAT204300.0135.718896
GTATCAA205200.0135.193769
TGGTATC205500.0135.066447
AAGCAGT205500.0135.065231
AGTGGTA209200.0132.545
CAGTGGT211900.0131.122884
AGCAGTG213200.0130.25472
GCAGTGG218700.0126.946053
TCAACGC202750.027.98307210-14
ATCAACG202450.027.94633710-14
CAACGCA204650.027.71624210-14
AGAGTAC203350.027.67401715-19
GTACATG203850.027.60616320-24
TACATGG203750.027.57731620-24
CAGAGTA204200.027.5658715-19
AGTACAT205500.027.33546820-24
CGCAGAG207100.027.26326415-19
GCAGAGT207650.027.13560315-19
GAGTACA203800.027.012520-24
Produced by FastQC (version 0.11.5)