FastQCFastQC Report
Sat 18 Mar 2023
SRR4895476_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895476_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173132
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGATG2178450.0143.540441
AGGATGA2180800.0143.385762
GGATGAT760150.0142.663393
GGATGAG609600.0142.32413
GGATGAA605100.0142.217323
GGATGAC221300.0142.040683
GATGACC59350.0137.95384
GATGATG237300.0137.072054
GATGAAT224000.0136.763374
GATGAAG186550.0136.566074
GATGATA235250.0136.339784
GATGACG58650.0136.288164
GATGAGT209900.0135.80454
GATGACT61350.0135.801994
GATGAGG199250.0135.083254
GATGAGA174400.0134.77674
GATGATT258500.0134.542114
GATGATC68000.0133.313394
GATGAGC59050.0132.684464
ATGATAG77150.0132.330085
Produced by FastQC (version 0.11.5)