FastQCFastQC Report
Sat 18 Mar 2023
SRR4895476_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895476_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173132
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA224931.0350498727182702No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC23990.11039366223496778No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG23810.10956536464420936No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC22280.1025248351227629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA299750.0136.416088
GTGGTAT302250.0135.287736
AAGCAGT302850.0135.253371
TGGTATC303400.0134.798667
GTATCAA305750.0133.927359
AGTGGTA306000.0133.559235
CAGTGGT310600.0131.720254
AGCAGTG313850.0130.306492
GCAGTGG323200.0126.5590743
CGAGTAA950.004895091530.3109682
ATCAACG300300.027.79401410-14
TCAACGC300850.027.79104410-14
AGAGTAC301650.027.60320715-19
CAGAGTA302500.027.52564215-19
TACATGG303600.027.38480620-24
GTACATG304800.027.30060820-24
CAACGCA307250.027.24026710-14
AGTACAT305750.027.18752920-24
GAGTACA301850.026.9473920-24
GCAGAGT309350.026.93009415-19
Produced by FastQC (version 0.11.5)