FastQCFastQC Report
Sat 18 Mar 2023
SRR4895475_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895475_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235374
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCTA2244750.0143.289752
GTTCTAG669000.0143.031973
GTGTTCT2258100.0142.442611
GTTCTAT780700.0142.371733
GTTCTAA604050.0142.046163
GTTCTAC204600.0141.782443
TTCTAGA185200.0140.512544
TTCTATG258900.0140.418664
TCTAGTA71050.0140.044375
TTCTAGT233250.0139.358064
TCTATAG78800.0138.688085
TTCTAGG216950.0138.519974
TCTAGAG60950.0138.46245
TCTATGG82900.0138.251165
TCTATGA74700.0137.342995
TCTAGGT76500.0137.214955
TTCTATA229400.0137.117954
TCTAGAT66600.0137.086585
TCTAGTG75000.0136.985475
TTCTACG56100.0136.582264
Produced by FastQC (version 0.11.5)