FastQCFastQC Report
Sat 18 Mar 2023
SRR4895475_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895475_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235374
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA169290.7573229356698252No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG81550.36481590999984786No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC56360.2521278318527459No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT33610.1503551530974235No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT28070.1255718282488747No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA26310.11769842540890249No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC25710.11501431080436651No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC23780.10638040882644247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA301400.0135.813968
GTGGTAT303500.0134.921666
GTATCAA303900.0134.744089
AAGCAGT304300.0134.506291
TGGTATC305250.0134.266047
AGTGGTA311600.0131.39135
CAGTGGT312250.0131.279114
AGCAGTG312900.0130.878452
GCAGTGG320400.0127.8597643
TCAACGC300500.027.87639410-14
ATCAACG300350.027.81364310-14
AGAGTAC302750.027.56462915-19
CAGAGTA304900.027.40330115-19
TACATGG304400.027.3924820-24
CAACGCA306900.027.34665710-14
GTACATG305700.027.29012120-24
AGTACAT308650.027.00130520-24
GAGTACA303600.026.90986820-24
GCAGAGT310950.026.8747615-19
CGCAGAG311750.026.79194315-19
Produced by FastQC (version 0.11.5)