FastQCFastQC Report
Sat 18 Mar 2023
SRR4895474_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895474_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences703015
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCA704350.0143.62172
GTCTGTC705100.0143.468951
CTGTCAT238550.0143.324453
CTGTCAA186500.0143.241683
CTGTCAG210750.0143.180593
TGTCATG74700.0142.452744
TGTCATA69950.0141.943224
CTGTCAC71250.0141.473973
TGTCAGA57350.0141.38844
TGTCAAT62500.0141.134674
GTCAGAG18200.0141.130255
TGTCAGG73000.0141.039264
TGTCAAG60700.0140.578614
GTCATAG22800.0140.42635
GTCATTA20900.0139.766485
TGTCAGT68900.0139.616094
GTCATGA20150.0139.612725
GTCAGTA21600.0139.567265
GTCATAT22950.0139.508485
GTCATAA20900.0139.422235
Produced by FastQC (version 0.11.5)