FastQCFastQC Report
Sat 18 Mar 2023
SRR4895474_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895474_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences703015
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34510.4908856852272No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC14400.20483204483545872No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG14170.20156042189711457No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT10210.14523160956736342No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT7110.10113582213750773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT108700.0139.02876
GGTATCA108600.0139.024198
TGGTATC109750.0137.764147
GTATCAA109850.0137.638759
AAGCAGT110200.0137.381061
AGTGGTA111600.0135.415955
CAGTGGT111850.0135.241964
AGCAGTG114100.0132.74842
GCAGTGG116400.0130.001663
TCAACGC108500.028.4008710-14
ATCAACG108600.028.34821110-14
AGAGTAC109750.028.10362415-19
GTACATG109950.028.06048620-24
CAGAGTA110000.028.03975315-19
TACATGG110350.027.91963420-24
CAACGCA110900.027.78624210-14
AGTACAT110900.027.75520320-24
GAGTACA109650.027.61206620-24
GCAGAGT112650.027.3545915-19
CGCAGAG112800.027.29269215-19
Produced by FastQC (version 0.11.5)