FastQCFastQC Report
Sat 18 Mar 2023
SRR4895473_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895473_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008372
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGA1009200.0143.774192
GTCGTAG1009450.0143.738571
CGTAGAT338250.0143.661393
CGTAGAG290300.0143.424483
CGTAGAA277700.0143.32543
CGTAGAC105300.0143.075453
GTAGATG109500.0141.464264
GTAGATA98150.0141.403054
GTAGAGT87200.0141.007574
GTAGAAT99700.0140.575824
GTAGAAG91550.0140.516084
GTAGAGA80300.0140.132234
GTAGAGG99100.0140.047524
GTAGACT25700.0138.856234
GTAGATT100350.0138.661514
GTAGACC28800.0138.649194
GTAGACG31250.0138.600044
TAGATAG33450.0137.227575
TAGATGG34800.0136.45255
GTAGAGC30950.0135.991584
Produced by FastQC (version 0.11.5)