FastQCFastQC Report
Sat 18 Mar 2023
SRR4895473_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895473_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008372
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52730.5229220962105254No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG29870.296220045776757No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC24790.24584181234703065No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC11930.11830951275918014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC10140.10055812735776082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA176400.0136.914468
GTGGTAT177050.0136.533756
AAGCAGT177550.0136.108671
TGGTATC178500.0135.384327
GTATCAA179000.0135.046369
AGTGGTA181050.0133.477515
AGCAGTG181350.0133.177282
CAGTGGT182500.0132.495864
GCAGTGG187150.0129.088423
CAACGCA177500.027.88622710-14
ATCAACG176950.027.87529210-14
TCAACGC177500.027.861910-14
AGAGTAC177400.027.78189715-19
CAGAGTA177500.027.75813715-19
TACATGG177350.027.64237620-24
GTACATG178150.027.55057320-24
CGCAGAG179750.027.3946615-19
AGTACAT179200.027.37307520-24
AACGCAG182200.027.19057510-14
GCAGAGT181300.027.16839415-19
Produced by FastQC (version 0.11.5)