FastQCFastQC Report
Sat 18 Mar 2023
SRR4895472_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895472_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617146
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAA617600.0143.77212
GTACGCA617700.0143.748831
ACGCAAT211950.0143.549153
CGCAATG70600.0143.277184
ACGCAAG187500.0142.929343
CGCAATA60000.0142.689514
CGCAAGT55500.0142.59234
CGCAATT62550.0142.278344
GCAATAG19000.0141.995325
ACGCAAA156550.0141.26913
CGCAAGA53400.0141.194054
CGCAAAT47250.0141.147864
ACGCAAC67050.0140.99153
GCAATGT24550.0140.665015
GCAATGA18950.0140.092065
GCAATAT20600.0140.04695
CGCAACT16150.0139.879324
CGCAAGG64650.0139.771124
CGCAAAG58050.0139.055114
GCAAGTA16100.0138.973135
Produced by FastQC (version 0.11.5)