FastQCFastQC Report
Sat 18 Mar 2023
SRR4895472_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895472_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617146
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28870.4677985436185279No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG26170.4240487664183191No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC17200.27870228438651473No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT10960.17759168819047683No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC10250.16608711714894045No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA8580.1390270697695521No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT7980.1293048970583946No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA7940.1286567522109841No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA7710.12492991933837373No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT7340.11893457949982662No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC7110.11520774662721624No Hit
CACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAG6710.10872629815311126No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT6330.10256892210271153No Hit
GAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGT6240.10111059619603789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT121050.0137.144131
GGTATCA121350.0136.990778
TGGTATC122350.0135.988747
GTGGTAT122500.0135.704736
GTATCAA122800.0135.431819
CAGTGGT123900.0134.171364
AGTGGTA124250.0133.561745
AGCAGTG124850.0132.854582
GCAGTGG128200.0129.495323
GTCAGGT950.00488097230.325472
TCAACGC121200.028.1802110-14
CAACGCA121800.028.01775710-14
ATCAACG121200.028.01395610-14
AGAGTAC121650.027.8998515-19
TACATGG122000.027.85063220-24
GTACATG122550.027.7608820-24
CAGAGTA122400.027.74065615-19
CGCAGAG122000.027.7136315-19
AGTACAT122900.027.51781720-24
GCAGAGT123950.027.37053515-19
Produced by FastQC (version 0.11.5)