FastQCFastQC Report
Sat 18 Mar 2023
SRR4895471_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895471_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936642
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA937850.0143.708592
GGTGCGA938150.0143.662631
TGCGAAG268800.0143.605943
TGCGAAT330200.0143.508163
GCGAAGT78850.0142.896644
GCGAAAT78850.0142.896644
GCGAATA94950.0142.839514
TGCGAAA241850.0142.829393
CGAATTG32050.0142.777925
GCGAATG106450.0142.751344
GCGAATT95750.0142.247224
TGCGAAC100200.0142.177033
CGAATAG31200.0142.05555
CGAAGTA24350.0141.8325
CGAATGA29100.0141.427875
GCGAAGA74250.0140.79954
CGAATTA26300.0140.343925
CGAATGT37600.0139.88195
CGAATAT31950.0139.846865
CGAAGTG24550.0139.797325
Produced by FastQC (version 0.11.5)