FastQCFastQC Report
Sat 18 Mar 2023
SRR4895471_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895471_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936642
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50850.5428968592055449No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG25730.2747047431142315No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC17530.18715795362582502No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA17460.18641060298385081No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT14160.15117835843363847No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC10980.11722728641252475No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCGGTGGCTCAC10620.11338376882522884No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT10090.10772525682170989No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA9590.10238703795046561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC201100.0138.104377
GGTATCA200950.0138.02848
GTGGTAT201350.0137.968646
AAGCAGT200900.0137.94991
GTATCAA201700.0137.515159
AGTGGTA205050.0135.514195
CAGTGGT205800.0135.020324
AGCAGTG205450.0134.999942
GCAGTGG209300.0132.585463
TCAACGC199900.028.29071810-14
ATCAACG199950.028.21885510-14
CAACGCA201200.028.11507610-14
AGAGTAC201450.028.03017215-19
CAGAGTA202000.027.98948115-19
TACATGG201550.027.94367420-24
CGCAGAG202900.027.80857515-19
GTACATG202550.027.79860720-24
AGTACAT203600.027.63403120-24
AACGCAG205150.027.58076110-14
GCAGAGT205850.027.48697315-19
Produced by FastQC (version 0.11.5)