FastQCFastQC Report
Sat 18 Mar 2023
SRR4895470_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895470_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617988
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAGC151.2667845E-4143.924848
AGAACAT201200.0142.959143
GGAGAAC622300.0142.918761
GAGAACA622950.0142.769652
GAACATA57100.0142.286454
GAACAGT60100.0142.128774
GAACATG65400.0141.834184
AGAACAG196600.0141.618823
GAACATT60200.0140.697284
GAACAAT55350.0140.284454
GAACATC20750.0139.416354
AACATAG20050.0138.900025
AGAACAC73150.0137.136863
AGAACAA163450.0136.440223
GAACAGA56750.0136.189674
GAACAAG55750.0136.180014
GAACACT19450.0135.785134
AACATGA17550.0135.313955
GAACAGG68400.0135.087364
AACATGG22500.0134.329835
Produced by FastQC (version 0.11.5)