FastQCFastQC Report
Sat 18 Mar 2023
SRR4895470_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895470_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617988
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37990.6147368557318265No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC13780.22298167601959906No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC9350.15129743619617209No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG8850.14320666420707198No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC8590.13899946277273992No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG8430.1364104157362279No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA7960.12880509006647378No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT7580.1226561033547577No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC6970.11278536152805554No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCGGGCGCGGTGGCTCA6380.10323825058091744No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA6350.1027528042615714No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA6280.1016200961830974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT111350.0139.026151
GGTATCA111150.0139.022848
GTATCAA111750.0138.405239
GTGGTAT111850.0138.345836
TGGTATC112250.0137.852847
AGTGGTA113150.0136.819965
CAGTGGT114150.0135.621354
AGCAGTG114650.0134.898862
GCAGTGG116600.0132.642843
GCGTGTA450.00991973547.9813358
ATCAACG111350.028.23293310-14
TCAACGC111750.028.22204210-14
CAGAGTA111650.028.1441815-19
AGAGTAC111900.028.08130515-19
TACATGG112000.028.04927820-24
GTACATG112550.027.91220920-24
CAACGCA113100.027.88517410-14
GCAGAGT113150.027.77107815-19
AGTACAT113200.027.66290720-24
GAGTACA111600.027.59507620-24
Produced by FastQC (version 0.11.5)