FastQCFastQC Report
Sat 18 Mar 2023
SRR4895469_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895469_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003185
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTA2004850.0143.783482
GCTCGGT2007850.0143.568651
TCGGTAA538800.0143.478163
TCGGTAG609200.0143.468493
TCGGTAT635450.0143.407273
TCGGTAC226650.0143.08013
CGGTATA183850.0142.92714
CGGTAGT195000.0142.392654
CGGTATG209800.0142.362184
CGGTAAG175250.0142.098984
CGGTAGA166650.0142.09214
CGGTAAT178900.0141.733644
CGGTAGG203950.0141.47124
CGGTATT195250.0141.25224
CGGTACG63650.0141.079384
CGGTACT60100.0138.757484
CGGTAAA154250.0138.0284
CGGTACA53250.0137.824984
CGGTAGC51550.0137.764044
CGGTAAC42000.0137.052864
Produced by FastQC (version 0.11.5)