FastQCFastQC Report
Sat 18 Mar 2023
SRR4895469_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895469_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003185
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG174320.87021418391212No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC117790.5880135883605359No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81820.40844954410101914No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT24880.12420220798378583No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT21180.10573162239134179No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC20600.10283623329847218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT412800.0138.92721
GGTATCA414050.0138.379658
GTGGTAT414800.0138.077416
TGGTATC416400.0137.667837
GTATCAA417700.0137.273839
CAGTGGT420400.0136.340854
AGCAGTG423550.0135.35012
AGTGGTA428250.0133.757635
GCAGTGG433050.0132.314353
TCAACGC413500.028.28363810-14
ATCAACG412800.028.27232410-14
CAACGCA417200.028.04315410-14
AGAGTAC414850.028.0293615-19
TACATGG416250.027.9401120-24
CAGAGTA416950.027.91235515-19
GTACATG419000.027.76016820-24
CGCAGAG419800.027.70228815-19
GCAGAGT420450.027.65261315-19
AGTACAT420850.027.59024420-24
GAGTACA415050.027.4797620-24
Produced by FastQC (version 0.11.5)