FastQCFastQC Report
Sat 18 Mar 2023
SRR4895468_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895468_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3062
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA812.6453298497713913No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCGGTGGCTCAC240.7838014369693012No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATGCAATCGGTTTAAACAT210.6858262573481384No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATTCGAAACTGGATGTGTA110.3592423252775963No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCTGTTTGCTCAGCCGC110.3592423252775963No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA100.32658393207054215No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGTGGTGGCTCA60.1959503592423253No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA50.16329196603527107No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGGGCTGAAGCATGGGCTG50.16329196603527107No Hit
ACCACCTTTTTTTCCTTTTTAATTAATACTAAATATTGTGATTTCTTATT50.16329196603527107No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT50.16329196603527107No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCCGGGCGCGGTGGCTC40.13063357282821686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCAGCCAGACTCTCCTAC40.13063357282821686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA40.13063357282821686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGAGTCCCGGTGCCCTC40.13063357282821686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT40.13063357282821686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGAGCGGCCAACATGG40.13063357282821686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC1450.0144.07
AGTGGTA1450.0144.05
GTATCAA1500.0144.09
AATTAAA100.00689151144.01
AGCAGTG1450.0144.02
GTGGTAT1450.0144.06
CAGTGGT1450.0144.04
GGTATCA1500.0144.08
GCAGTGG1500.0139.23
AAGCAGT1500.0139.21
ATGGGAG255.126132E-428.80000125-29
AACGCAG1500.028.80000110-14
ACGCAGA1500.028.80000115-19
GTACATG1500.028.80000120-24
TCAACGC1500.028.80000110-14
TACATGG1500.028.80000120-24
CAGAGTA1500.028.80000115-19
AGTACAT1500.028.80000120-24
AGAGTAC1500.028.80000115-19
GAGTACA1500.028.80000120-24
Produced by FastQC (version 0.11.5)