FastQCFastQC Report
Sat 18 Mar 2023
SRR4895467_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895467_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328241
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATC1331250.0143.574782
CCTCTAT1332100.0143.483171
TCTATCG420700.0142.86123
CTATCGA105600.0142.746174
TCTATCA332150.0142.561423
CTATCAG107500.0142.030384
TCTATCT390000.0141.838183
CTATCGG130250.0141.805314
CTATCAT116300.0141.8014
CTATCGT150350.0141.607384
CTATCTA102550.0141.589114
TCTATCC200400.0141.534823
CTATCTG127650.0141.255264
TATCGAG33450.0140.893045
TATCGTA42950.0140.553995
CTATCCG51550.0140.275484
CTATCAA88450.0139.51174
TATCGAT37200.0139.455835
CTATCCA52700.0139.125894
TATCGGG43250.0139.079965
Produced by FastQC (version 0.11.5)