FastQCFastQC Report
Sat 18 Mar 2023
SRR4895467_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895467_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328241
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57430.43237635338767594No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG35420.2666684735676733No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC24350.18332516463503234No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT16780.1263324953829915No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT15480.11654511493019715No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA13440.10118645637350451No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC13400.10088530620572622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA267800.0137.839518
AAGCAGT268600.0137.823971
TGGTATC268250.0137.661937
GTGGTAT269150.0137.281836
GTATCAA270100.0136.639119
AGTGGTA271400.0136.143725
CAGTGGT272450.0135.856784
AGCAGTG275150.0134.516892
GCAGTGG281550.0131.433583
ATGCGTC450.00992330547.9800156
TCAACGC267800.028.15376910-14
ATCAACG267200.028.13618310-14
AGAGTAC269350.027.9062515-19
CAGAGTA270600.027.77202215-19
CAACGCA272050.027.72981810-14
GTACATG271500.027.69047420-24
TACATGG271650.027.67518420-24
AGTACAT272850.027.51653120-24
CGCAGAG274100.027.4541615-19
AACGCAG275900.027.34286710-14
Produced by FastQC (version 0.11.5)