FastQCFastQC Report
Sat 18 Mar 2023
SRR4895466_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895466_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434956
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAATCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC6600.1517394862928664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCCG159100.0143.635423
CTAATCC435900.0143.593142
CCTAATC436150.0143.510831
AATCCGT51450.0142.648124
ATCCGAT16250.0142.57845
TAATCCT138250.0142.501533
AATCCGA39850.0142.101174
AATCCGG53650.0141.760914
TAATCCA86650.0141.581683
ATCCGGA13400.0141.221955
AATCCTA42250.0141.181924
AATCCTG45550.0140.747474
ATCCGTA13950.0140.29625
ATCCTAG14150.0139.838735
ATCCAGT10400.0139.755625
ATCCGTG16300.0139.492455
AATCCAT30850.0139.475284
TAATCCC55750.0139.389513
AATCCAG27950.0139.272924
ATCCATA8750.0138.972825
Produced by FastQC (version 0.11.5)