FastQCFastQC Report
Sat 18 Mar 2023
SRR4895465_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895465_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1649983
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAC1654300.0143.510442
CCGACAA1656400.0143.328491
GACAACT490000.0143.169343
GACAACG518600.0142.987053
ACAACGT170650.0141.485724
ACAACTG162950.0140.842354
GACAACA435950.0140.835753
ACAACTA139800.0140.132034
CAACGTA50700.0139.347925
ACAACGA142300.0139.338554
CAACTGA43900.0139.300065
CAACGTG54700.0139.28545
CAACGAT50600.0139.196765
CAACTAG46350.0138.766535
CAACGTT55050.0138.661225
CAACGGT55900.0138.48335
ACAACGG171850.0138.404514
GACAACC229350.0138.24243
ACAACAT148950.0138.092674
CAACTAT47400.0137.362185
Produced by FastQC (version 0.11.5)