FastQCFastQC Report
Sat 18 Mar 2023
SRR4895465_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895465_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1649983
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69840.4232770883093947No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG33020.20012327399736846No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC25520.1546682602184386No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA20820.12618311825030926No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT20310.12309217731334202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT264450.0138.165311
GGTATCA265950.0137.350638
GTGGTAT266350.0137.063316
TGGTATC267250.0136.655587
GTATCAA269150.0135.797849
AGTGGTA269650.0135.385935
CAGTGGT271250.0134.773044
AGCAGTG273800.0133.368212
GCAGTGG277600.0131.672243
TCAACGC265400.028.2049310-14
ATCAACG265700.028.08641210-14
AGAGTAC266050.028.01261515-19
CAGAGTA267650.027.84515615-19
TACATGG267550.027.76613820-24
GTACATG268100.027.72528520-24
AGTACAT269750.027.5663720-24
CAACGCA273450.027.41145510-14
GCAGAGT272150.027.39002815-19
GAGTACA266800.027.26140420-24
CGCAGAG275900.027.10121215-19
Produced by FastQC (version 0.11.5)