FastQCFastQC Report
Sat 18 Mar 2023
SRR4895464_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895464_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726977
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCCTCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC7930.10908185540945588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCCTC729850.0143.35652
ATCCTCG231100.0143.212193
CCATCCT730750.0143.179951
ATCCTCA184100.0142.951163
ATCCTCT219600.0141.536153
TCCTCGA60300.0141.422184
CCTCGTA21600.0140.263575
TCCTCGG80150.0140.067574
CTCGGTA8750.0139.816166
TCCTCAG58600.0139.138984
TCCTCGT76150.0138.919744
CCTCGAT20200.0138.584525
ATCCTCC102050.0138.498473
TCCTCTA62600.0138.180474
CTCGATA6000.0137.931386
TCCTCTG72800.0137.503024
CCTCGAG20850.0137.370515
TCCTCAA54200.0136.891314
CCTCAGT19850.0136.67765
CTCGAAT5800.0136.483836
Produced by FastQC (version 0.11.5)