FastQCFastQC Report
Sat 18 Mar 2023
SRR4895464_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895464_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726977
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37040.5095071783563992No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG28040.38570683804301925No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG21220.2918936912722136No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC19960.27456164362834035No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC9330.1283396861248705No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG8240.11334608935358341No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC7810.10743118420527746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT178300.0135.945361
GTGGTAT180350.0134.135836
GGTATCA180350.0134.056028
TGGTATC181050.0133.617237
GTATCAA181800.0133.184779
AGTGGTA182650.0132.407335
CAGTGGT183150.0132.163774
AGCAGTG183100.0132.145542
GCAGTGG188100.0128.709473
CGCACGA200.00628059128.80458360-64
TCAACGC180350.027.45773710-14
ATCAACG180100.027.4159210-14
GTACATG180800.027.29039620-24
AGAGTAC181200.027.26537915-19
TACATGG180950.027.243920-24
AGTACAT181100.027.20543920-24
CAACGCA182300.027.17192810-14
CAGAGTA182000.027.11389415-19
CGCAGAG183650.026.8624515-19
GCAGAGT183950.026.81081415-19
Produced by FastQC (version 0.11.5)