FastQCFastQC Report
Sat 18 Mar 2023
SRR4895463_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895463_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258900
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCTTACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC13090.10397966478671856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAC1263100.0143.433782
CACCTTA1264700.0143.252321
CCTTACG361300.0143.016083
CTTACGA94100.0142.230014
CTTACGT126700.0141.981374
CTTACGG113800.0141.952164
CCTTACA340200.0141.670293
CTTACTG125350.0141.15694
CTTACTA115400.0140.981674
CTTACAG101300.0140.786874
CCTTACT405250.0140.680713
TTACGAT35650.0139.673655
TTACGTA39000.0138.746235
CCTTACC176100.0137.94663
TTACGGT41750.0137.880055
TTACGGA30800.0137.838095
TACGATG10450.0137.715136
TTACGAG28250.0137.544515
CTTACCG43600.0137.475864
CTTACAT121550.0137.341234
Produced by FastQC (version 0.11.5)