FastQCFastQC Report
Sat 18 Mar 2023
SRR4895463_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895463_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258900
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60030.47684486456430214No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG44950.3570577488283422No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC40230.31956469934069426No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT21390.1699102390976249No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC16380.13011359123043928No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG13460.10691873858130115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA215900.0138.007988
AAGCAGT217950.0136.92941
GTGGTAT217750.0136.901576
TGGTATC218800.0136.244587
GTATCAA219100.0135.95959
AGTGGTA220750.0135.041085
CAGTGGT221850.0134.468834
AGCAGTG222650.0134.006562
GCAGTGG227350.0131.267913
ATCAACG216200.028.06263210-14
TCAACGC217250.028.0197610-14
TACATGG217300.027.81326720-24
AGAGTAC217750.027.79016115-19
CAGAGTA219350.027.62682215-19
GTACATG219900.027.5171520-24
CAACGCA222050.027.42054410-14
GAGTACA217150.027.31534420-24
AGTACAT221750.027.20967320-24
CGCAGAG223800.027.0839315-19
GCAGAGT224600.027.04513715-19
Produced by FastQC (version 0.11.5)