FastQCFastQC Report
Sat 18 Mar 2023
SRR4895462_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895462_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765212
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGAGCT532250.0143.101433
AAGGAGC1775100.0143.082932
GGAGCTA142000.0142.669464
CAAGGAG1785250.0142.269421
AGGAGCG535600.0142.246673
AGGAGCA456900.0141.743963
GGAGCAT153700.0141.545034
GGAGCGT183550.0141.180924
GAGCTAT50200.0140.875845
GAGCTAG44900.0140.841055
GAGCTTA44850.0140.837665
GGAGCTT166150.0140.204914
GAGCGTA51850.0139.861595
GGAGCTG173250.0139.691334
AGGAGCC263950.0139.687943
GGAGCGA143250.0139.666764
GAGCGAT49100.0139.343195
GAGCATG50800.0139.211945
GAGCATA44250.0139.007925
GAGCAGT47600.0138.595495
Produced by FastQC (version 0.11.5)