FastQCFastQC Report
Sat 18 Mar 2023
SRR4895462_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895462_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765212
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70060.39689283780078544No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG59770.3385995563139158No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC34730.1967469063206006No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC31160.1765227066210744No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC25820.14627138270077475No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT24800.14049303992948156No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA23680.13414819296492433No Hit
TGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCA19940.11296093613684928No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA19680.1114880252343628No Hit
GGCAGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCG18740.10616288581768082No Hit
AGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGG18600.10536977994711116No Hit
GAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGT18510.10485992617317354No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA18280.10355696652866624No Hit
GTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAG18080.10242395814213817No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT17820.10095104723965166No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT17780.10072444556234604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT332500.0138.444891
GGTATCA333250.0138.119848
GTGGTAT333850.0137.785396
TGGTATC335450.0137.192557
GTATCAA335600.0137.088339
AGTGGTA338600.0135.8955
CAGTGGT341900.0134.709644
AGCAGTG342950.0134.205342
GCAGTGG349350.0131.684923
TCAACGC333150.028.25437410-14
ATCAACG333700.028.13016910-14
AGAGTAC334250.028.02024515-19
CAGAGTA335000.027.98329415-19
GTACATG336700.027.88742820-24
CAACGCA338250.027.8326210-14
TACATGG337850.027.75841120-24
AGTACAT337800.027.69859120-24
GCAGAGT341200.027.48323815-19
CGCAGAG341300.027.44566515-19
GAGTACA334950.027.40988520-24
Produced by FastQC (version 0.11.5)