FastQCFastQC Report
Sat 18 Mar 2023
SRR4895461_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895461_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293443
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCT1296550.0143.537841
TGGCTCA318400.0143.322543
TTGGCTC1298650.0143.305742
TGGCTCG363400.0142.897553
TGGCTCT414750.0142.499733
GGCTCGA96700.0142.176274
GGCTCAG99350.0141.7154
GGCTCTA111950.0141.638184
GGCTCAT108350.0141.629854
GGCTCGT118150.0140.903734
TGGCTCC209250.0140.689933
GCTCTAG35600.0140.6545
GCTCGAT37900.0140.470575
GGCTCTG138000.0140.394524
GCTCAGA26850.0140.136185
GCTCATA29500.0139.741525
GCTCATG34150.0139.674035
GCTCGAA25900.0139.443025
GGCTCGG120900.0139.424424
GCTCAGT34700.0139.118825
Produced by FastQC (version 0.11.5)