FastQCFastQC Report
Sat 18 Mar 2023
SRR4895461_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895461_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293443
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49910.3858693425222449No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG49000.37883385661370467No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC45030.3481405829247984No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA20350.15733202004263042No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC14270.11032569660974624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA202950.0137.334828
GTGGTAT204200.0136.529376
TGGTATC205150.0136.037467
GTATCAA205400.0135.766789
AAGCAGT206000.0135.289121
AGTGGTA206800.0134.673665
CAGTGGT208850.0133.386224
AGCAGTG209050.0133.211962
GCAGTGG213500.0130.334243
ATCAACG202200.028.03864710-14
TCAACGC203150.027.95003710-14
AGAGTAC202800.027.85052715-19
GTACATG203700.027.71977420-24
CAGAGTA203800.027.71387115-19
AGTACAT204800.027.549820-24
TACATGG205600.027.42859620-24
CAACGCA207400.027.39811110-14
GAGTACA203400.027.22979520-24
CGCAGAG209250.027.04708515-19
GCAGAGT209200.027.03290615-19
Produced by FastQC (version 0.11.5)