FastQCFastQC Report
Sat 18 Mar 2023
SRR4895460_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895460_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences858090
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATTCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC11550.13460126560151034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTC860100.0143.565571
TCATTCC861700.0143.2992
CATTCCG288650.0143.011183
CATTCCT268300.0141.763093
CATTCCA190250.0141.60153
ATTCCGA75150.0141.51494
ATTCCGT99600.0141.235574
ATTCCTA80500.0140.869524
ATTCCGG90200.0140.637944
ATTCCTG80050.0139.594014
TCCGATG10350.0137.651676
TTCCGAT31300.0137.471775
ATTCCAT68100.0137.463334
ATTCCAG60800.0136.452794
TCCGATA8850.0135.778156
CATTCCC127250.0135.37023
ATTCCGC28850.0134.929894
TCCGAAT8350.0134.429576
TCCGTAG8200.0134.256166
TTCCGTA27150.0133.57265
Produced by FastQC (version 0.11.5)