FastQCFastQC Report
Sat 18 Mar 2023
SRR4895460_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895460_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences858090
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG50960.593877099138785No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC31300.3647636028854782No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23310.2716498269412299No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA19150.2231700637462271No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC9540.11117714925007866No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT9420.1097786945425305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT225750.0139.531131
TGGTATC227450.0138.311987
GTGGTAT227500.0138.281596
GGTATCA227250.0138.275368
GTATCAA228750.0137.463039
CAGTGGT231100.0136.34554
AGTGGTA231500.0135.892295
AGCAGTG232050.0135.805052
GCAGTGG235800.0133.584213
CGTGGTA700.00109522341.126995
TCAACGC227300.028.4152610-14
ATCAACG227200.028.35807210-14
CAACGCA228200.028.30319210-14
AGAGTAC228350.028.1963515-19
CAGAGTA229200.028.1106215-19
GTACATG229250.028.07172420-24
TACATGG229200.028.0464420-24
CGCAGAG229950.028.03145415-19
GCAGAGT230700.027.94656615-19
AGTACAT230300.027.92498420-24
Produced by FastQC (version 0.11.5)