FastQCFastQC Report
Sat 18 Mar 2023
SRR4895459_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895459_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775864
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAACG1777100.0143.790251
CTAACGA1777300.0143.774082
TAACGAT564700.0143.687623
TAACGAG563650.0143.4833
TAACGAA455650.0143.180923
TAACGAC197550.0143.126663
AACGATG191050.0142.950014
AACGATA160900.0142.460594
AACGAGT168150.0142.008854
AACGAAT155600.0141.903244
ACGATGT61800.0141.44675
AACGATT171550.0141.417084
ACGATAG55250.0141.28715
ACGATGG66100.0140.734995
AACGATC47250.0140.693864
ACGATGA51450.0140.535395
AACGAAG155450.0140.512864
ACGAGTG55350.0140.51195
ACGAAGT50100.0140.157755
AACGACT49350.0140.101014
Produced by FastQC (version 0.11.5)